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DR. CLAIRE CARD ON ‘99 AI

This article was originally printed in the June / July 2000 issue of Tracking The Industry. . .


- This article is written in grateful acknowledgment of the contributions by Dr. Murray Woodbury, Specialized Livestock Chair, Ron and Cathy Friesen and the clients of the Western College of Veterinary Medicine.

The White Tailed Deer (WTD) industry is an emerging industry in Saskatchewan. As with many livestock industries there is a move towards increasing profitability and quality. The main products of the WTD industry are breeding animals and monster bucks. Breeding management strategies to make rapid improvements in genetic traits usually involve artificial insemination. The added advantages of this industry includes the ability to limit the introduction of disease by AI, as compared to the introduction of new animals.

A summary of the information presented to the White-tailed Deer Producers regarding synchronization of estrus (female receptivity) is as follows: There are no magic formulas. Methods that may be applied usually rely on programmed synchronization of receptivity. Methods that have been used include intravaginal devices (CIDR's) (from Australia and New Zealand), vaginal sponges, ear implants, and prostaglandin injections. The starting date of treatment is usually prior to the breeding season, late October or early November, or it may be close to the natural breeding season which appears to coincide with the full moon in November. The hormonal devices are usually left in place for 8-14 days and then removed. Does are inseminated 36-72 hours following the withdrawal of the hormonal treatment.

Progestagen-containing vaginal sponges (Veramix, Upjohn) are available in Canada. A 10 and 12 day exposure period has been used. Sponges tend to fall out as the doe may get hold of the strings, which need to be cut very short, or the secretion in reaction to the sponges may soak them and they slide out when the doe urinates. Sponge fallout usually occurs near the end of the insertion period. The sponges are sprayed with an antibiotic spray or coated with Alban cream prior to insertion. They can be hard to remove. The insertion equipment for sheep may be too big for application in small or virgin does. At removal I suggest administration of prostaglandin for application in small or virgin does. At removal I suggest administration of prostaglandin (lutalyse, Upjohn, 10mg (2cc) IM). This will help synchronize does that the sponges fell out of and also may tighten the window of receptivity. In our experience 10/24 fell out (41%) over a two year period. Pregnancy data for 1998 was 58% (7/12), 2 does were lost to follow-up. Data on 10 more will be available in the spring. Of the does where the sponges fell out, many were bred and became pregnant. The external signs such as a swollen vulva, tarsal hair erection (inside of back legs at the hock), and internal signs of receptivity such as mucus production and a soft open cervix should be checked when deciding which does to breed. I suggest you synchronize more than you need. Breed at about 60 hours post withdrawal of sponge. The use of 2 insertions of sponges at 5 day intervals may increase the synchronization. Inadvertent placement of the sponge or CIDR into the urinary tract will make the doe strain and posture as if to urinate. Remove sponge or CIDR if these signs occur and treat with long acting goxytetracycline.

CIDR's - These t-shaped intravaginal devices are not presently available in Canada. A 10 day treatment period is most common. These devices deliver progesterone, a natural non-synthetic hormone, so residues from their use should not be a problem. Few if any CIDR's fall out. We used prostaglandin as above on the day of CIDR withdrawal. Breed at about 48-60 hours post withdrawal. Pregnancy results are variable. Data from this year, using a non-return rate to breeding to indicate pregnancy were 58% (14/24) in one group and 91% (10/11) in the second group. The combined group rate was 68.5% (24/35). The 58% group was also a group with a high proportion of does with a 1998 birth date. It is possible to have small injuries that bleed during insertion of the intravaginal devices. Many of the CIDR does developed a heavy vaginal discharge. Insemination was performed by avoiding the discharge.
Ear Implants are small hard brittle implants that were available in the US cattle (Synchromate B) but are not presently being manufactured, and may be coming to Canada through a European company (Crestar, Intervet) in a few years. Others report loss of implants, but we have not had any losses. We removed the plastic coating on the implants and then loaded one-half an implant into an implant gun from an equine product call Ovuplant. Implants are placed under the skin of the ear. We have non-return rate information on synchronized implanted does bred by a buck, compared with implanted and bred AI at 60 hours post withdrawal. Prostaglandin was given at withdrawal as above. Non-return rates were the same 93% (14/15) for synchronized does bed with a buck, and 93% (14/15) bred AI. Another implant and prostaglandin group bred Ai had a 71% (10/14) non-return rate. The combined AI group non-return rate was 24/29. Note the other half of the does in this pen were given CIDR's and are listed above at the 91% (10/11) rate mentioned above.

Prostaglandin (Lutalyse 10 mg, 2 cc, IM) has been used in a few animals that failed to respond to one of the above treatments. It appears that two shots may also be used 14 days apart with no other agents during the breeding season. The doe must be cycling if this product is to work. If a doe fails to respond to synchronization you may wait one week, give the prostaglandin and recheck 3 days later, breed if in estrus (receptive) at that time. Very high doses of prostaglandin may be used to cause abortion in does, so don't confuse it with a dewormer or antibiotic. In summary it appears the least desirable methods, intravaginal sponges and prostaglandin, are the most highly available in Canada. Sponges will provide synchronization but are probably better used in mid November and should be combined with prostaglandin. Prostaglandin is only effective when the does are in season (mid-November). There is no clear superiority of CIDR's versus ear plants in terms of synchronization of female receptivity. A few more years worth of data are required to get large enough numbers to perform these comparisons. Technically CIDR's are somewhat more difficult to insert and carry a minor risk of urinary tract injury. In the future we need to closely evaluate the profitability of AI. It has proven beneficial for other livestock groups to identify a short list of products they for animal health management. It is advisable as producers to identify these products and lobby the companies and government agencies for their help in getting approval for their safe and legal use.

written by Dr. Clair Card


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